Composite

Part:BBa_K3425056

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)


T7 polymerase regulated expression construct of Juniper GFP

BBa K3425056 is a T7 polymerase regulated expression construct of Juniper GFP constructed from the weaker promoter, BBa_K3425035, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
    Illegal BamHI site found at 670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None